Areas of Interest
Protein engineering, protein recovery, protein purification, drug delivery and vaccine development.
Ph.D. Chemical Engineering, Iowa State University, Ames, IA, 1999
M.S. Physical & Analytical Chemistry, Iowa State University, Ames, IA, 1996
M.S. Metallurgical Physical Chemistry, University of Science and Technology Beijing, China, 1991
B.S. Metallurgical Physical Chemistry, University of Science and Technology Beijing, China, 1986
2007 - present: Associate Professor, Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA
2001 - 2007: Assistant Professor, Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA
1999 - 2001: Scientist, Covance Biotechnology Services, Research Triangle Park, NC
1996 - 1999: Research Associate, Department of Chemical Engineering, Iowa State University, Ames, IA
1993 - 1997: Research Associate, Department of Chemistry, Iowa State University, Ames, IA
1991 - 1993: Product Manager and Engineer, Yitai New Technology Institute, Beijing, China
Teaching and Workshops
BSE 3524 Unit Operations in Bioprocess Engineering
BSE 4544 Protein Separation Engineering
BSE 5614 Advances in Recombinant Protein Production
Awards and Recognitions
Outstanding New Assistant Professor Award College of Engineering, Virginia Tech, 2004
NAE/IOM Young Investigator Travel Award (Vaccine-2006), 2006
National Academy of Engineering, Young Investigator Award/Vaccine, Production Starter Grant, 2006
Lakshmi P., D. Bevan, C. Zhang. 2008. Quantifying protein microstructure and
electrostatic effects on the change in Gibbs free energy of binding in immobilized metal affinity chromatography. Analytical Chemistry, 80(5): 1628-1640.
Matanin B., Y. Huang, X. Meng, C. Zhang. 2008. Purification of the major envelop
protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from
native virions. , Journal of Virological Methods, 147:127-135.
Holler C., C. Zhang. 2008. Purification of an acidic recombinant protein from
transgenic tobacco., Biotechnology & Bioengineering, 99(4):902-909.
Holler, C., Vaughan, D., and Zhang, C. 2007. Polyethyleneimine precipitation vs. anion
exchange chromatography in fractionating recombinant beta-glucuronidase from transgenic
tobacco extract. Journal of Chromatography A. 1142 98-105.
Pathange, L.P., Bevan, D.R., Larson, T.J., and Zhang, C. 2006. Correlation Between Protein
Binding Strength on Immobilized Metal Affinity Chromatography and the Histidine-Related
Protein Surface Structure. Analytical Chemistry. 78(13) 4443-4449.
Zhang, C. 2006. Protein recovery from tobacco extract by non-chromatographic methods.
BioProcessing Journal 5(1):19-23.
Zhang, C., Medina-Bolivar, F., Buswell, S., and Cramer, C.L. 2005. Purification and stabilization
of ricin B from tobacco hairy root culture medium by aqueous two phase extraction. Journal
of Biotechnology. 117(1) 39-48.
Zhang, C. and Glatz, C.E. 2005. Applicability of the stoichiometric displacement model to
description of the retention behavior of charged-fusion proteins during fast protein liquid
chromatography. Journal of Chromatography A. 1069(1) 113-118.
Zhang, C., Lillie, R., Cotter, J., and Vaughan, D. 2005. Lysozyme purification from tobacco
extract by polyelectrolyte precipitation. Journal of Chromatography A. 1069 (1) 107-
Buswell, S., Medina-Bolivar, F., Chen, Q., Van Cott, K., and Zhang, C. 2005. Expression
of porcine prorelaxin in transgenic tobacco. Annals of the New York Academy of Sciences
U.S. Patent 6,841,658 (2007). Purification of human Troponin I. The patent is being used by Diosynth Biotechnology to produce clinical trial materials.